SERVAGel™ N 4 - 16, Vertical Native Gel 4 - 16 % | precast gel, 12 sample wells | 10 gels – Witec AG

Art No | 43253.01
190.00 CHF
190.00 CHF
Brochure

Size: 10/Pack

Description

The SERVAGel™ N 4-16 can be operated with the Blue and Clear Native buffer systems.

Blue and Clear Native electrophoresis in polyacrylamide gels (BN/CN PAGE) separates proteins according to their native state, i.e. by their intrinsic charge and size.

Blue Native PAGE (BN PAGE) makes use of Coomassie® Brilliant Blue G 250 to bind to the outer surface of protein complexes leading to a negatively charged protein-dye complex. The Blue G dye does not act as a detergent thus maintaining the native structure of the protein throughout the electrophoresis process. At physiological pH, the protein-dye complexes migrate pI-independently towards the anode. The repulsion between the negatively charged protein-dye complexes leads to high resolution and band sharpness.

Clear Native PAGE (CN PAGE) works without using any anionic dye. Therefore, migration of proteins through the gel is as well dependent from the intrinsic charge of the protein. This method can be used for separation of proteins with pI <7 at physiological pH when dyes may interfere with further analytical methods.

HS: 38220000
Storage Temperature: +2 °C to +8 °C (will be delivered cooled on blue ice)

Supplier

Serva Electrophoresis GmbH
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Accessories

40.00 CHF
40.00 CHF
Art No | 42538.01
SERVA Blue G solution for BN, 1%

Size: 20ml

38.00 CHF
38.00 CHF
74.00 CHF
74.00 CHF
130.00 CHF
130.00 CHF
Art No | 42534.01
Sample Buffer for Clear Native (2x) - 20ml

Size: 20ml

20 ml

40.00 CHF
40.00 CHF
Art No | 39219.01
SERVA Native Marker, liquid mix

Size: 5x 50 µL

Application: Blue Native and Clear Native gel electrophoresis

230.00 CHF
230.00 CHF
Art No | 43204.01
SERVAGel™ N Native Starter Kit

Size: 1 Kit

150.00 CHF
150.00 CHF

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