The SERVAGel™ N 4-16 can be operated with the Blue and Clear Native buffer systems.
Blue and Clear Native electrophoresis in polyacrylamide gels (BN/CN PAGE) separates proteins according to their native state, i.e. by their intrinsic charge and size.
Blue Native PAGE (BN PAGE) makes use of Coomassie® Brilliant Blue G 250 to bind to the outer surface of protein complexes leading to a negatively charged protein-dye complex. The Blue G dye does not act as a detergent thus maintaining the native structure of the protein throughout the electrophoresis process. At physiological pH, the protein-dye complexes migrate pI-independently towards the anode. The repulsion between the negatively charged protein-dye complexes leads to high resolution and band sharpness.
Clear Native PAGE (CN PAGE) works without using any anionic dye. Therefore, migration of proteins through the gel is as well dependent from the intrinsic charge of the protein. This method can be used for separation of proteins with pI <7 at physiological pH when dyes may interfere with further analytical methods.
Storage Temperature: +2 °C to +8 °C (will be delivered cooled on blue ice)
SupplierServa Electrophoresis GmbH
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Size: 5x 50 µL
Application: Blue Native and Clear Native gel electrophoresis
Size: 1 Kit