Size: 10 purifications
For the purification of genomic DNA from 1-50 mg plant tissue yielding up to 10 µg highly purified DNA (depending on plant and tissue age) suitable for all molecular biology applications.
For single-step spin column purification of genomic DNA from up to 50 mg of plant tissue (leaves, soft roots, seeds, fruit tissue, bud & blossom tissue).
- High DNA yield: up to 5-fold more than Silica plant kits
- High purity: reliable removal of inhibitors for downstream reactions (e. g., PCR, NGS)
- Convenience: no tedious bind-wash-elute procedure, grinding solution enables
- faster disruption of samples
- Speed: Whole process complete in 45 min
- Sustainability: 70 % less plastic than with Silica-based methods, no hazardous liquids
Kit contents (10 purifications):
- 10 EchoLUTION spin columns
- Plant Lysis Buffer, TurboLyse P Protease
- RNase A, Grinding Suspension
- Clearing Solution, Tris Buffer (10x)
Technology & Details
The EchoLUTION Plant DNA Kit has been developed to quickly purify genomic DNA from various species and types of tissues, including leaf, seed, fruit and root material (see table). No optimization of parameters like lysis time, temperature or elution volume is necessary.
Grinding solution (provided) enables a fast and convenient disruption of plant tissue when not using bead beating. The subsequent lysis step takes place under physiological conditions where the enzyme activity of proteases is generally increased. BioEcho’s TurboLyse P Protease Mix (patent pending) contains a unique combination of several active enzymes with an expanded substrate specificity. All kinds of plant tissues can be lysed fast and successfully – simply and with the same protocol. BioEcho also provides tissue grinding pestles supporting the initial mechanical disruption step in a 1.5 ml reaction tube.
The subsequent EchoLUTION single-step spin purification procedure combines an initial filtration, holding back potential cell debris, with an extremely efficient reverse purification. The cleared lysate is directly loaded onto the spin column. After just one single spin, the purified high molecular weight genomic and mitochondrial DNA is eluted, while all debris and cellular compartments, inhibitors, small fragments (< 50 bp), and salts are completely removed. Since impurities are held back by the purification matrix and since the highly pure DNA is collected in the flow-through, the complete DNA is easily recovered with close to 100 % recovery and highest purity. Addition of carrier nucleic acids is not required since matrix interactions have been minimized.
The extraction efficiency of the EchoLUTION Plant DNA Kit process is up to 5 fold higher than with common Silica-based purification kits (see below).
Yield of genomic DNA from leaf tissue of different plant species – EchoLUTION Plant DNA Kit compared to a Silica kit (DNeasy Plant Mini kit, Qiagen).
Corresponding volumes of elution fractions were loaded (BioEcho, 1ul; Q, 2ul). The BioEcho kit provides consistently higher yields. M, DNA size ladder (Thermo GeneRuler 1kb Plus).
SupplierBioEcho Life Sciences GmbH
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