Size: 8 x 384-well plate
For single-step 384-well plate-based purification of genomic DNA from up to 50 mg plant tissue (leaves, soft roots, seeds, fruit tissue, bud & blossom tissue); lysis is performed in 96-well plates (not contained in this kit!)
Genomic research and DNA analyses such as PCR and NGS require high-quality genomic DNA from quick procedures, no matter what the sample amount or source. The EchoLUTION Plant DNA Kits have been developed to obtain high-purity genomic DNA from all kinds of tissue and from amounts of starting material up to 30 mg. The purified DNA comprises of long DNA fragments, free of contaminants and enzyme inhibitors like chaotropic reagents and organic solvents and is highly suitable for all downstream applications.
The EchoLUTION Plant DNA Kits provide:
- Highly efficient extraction & high DNA yield – nearly 100 % recovery, even from difficult plant materials
- Reliable downstream performance in PCR and NGS – no re-work on samples
- High detection sensitivity due to greater purity – no carry-over of inhibitors
- Fast and convenient process – complete in less than 60 min, no tedious bind-wash-elute procedure
- Sustainability at lower costs – 70 % less plastic, no hazardous liquids, plastic-free packaging
Consistently high yields from all plant tissue types
Extraction of DNA from plant material typically starts with mechanical disruption of the plant tissue (e. g., bead-beating, mortar/pestle). The EchoLUTION Plant workflow then starts with the lysis step with TurboLyse enzymes: it takes place under physiological conditions where the enzyme activities are maximal. All kinds of tissue can be lysed fast and successfully – simple and with the same protocol. The subsequent EchoLUTION single-step spin purification procedure combines an initial filtration, holding back potential cell debris, with an extremely efficient reverse purification. The cleared lysate is directly loaded onto the spin column. After just one single spin, the purified genomic DNA is eluted with high yields while all impurities and salts are completely removed.
Highest Purity – Maximal Reliability
The EchoLUTION Plant DNA procedure delivers high-quality DNA:
- High DNA fragment size: 30-50 kb
- Text book DNA purity: A260/280 1.9
- Absence of amplification-inhibiting impurities
Downstream applications such as qPCR and NGS work reliably, re-work on single preparations is a thing of the past.
The EchoLUTION workflow
is based on aqueous solutions only – chaotrophic reagents such as phenol, guanidine-hydrochloride (Gu-HCl), or organic solvents are omitted.
Since wash steps are never complete, Silica preps consequently contain trace amounts of these substances as impurities whereas EchoLUTION samples are highly pure. This is confirmed spectrophotometrically, where BioEcho elution fractions typically show values within the optimum range.
In addition, the EchoLUTION process removes all interfering substances originating from the sample which results in maximum purity and reliable OD readings.
Unmatched DNA extraction performance in High Throughput
For applications in plant research and molecular breeding, the EchoLUTION Plant DNA workflow can also be performed in high throughput. The process delivers the same high-quality DNA as from single reactions but within a fraction of the time and with only three centrifugation steps.
Following the lysis step, the lysate is centrifuged and the supernatant loaded onto the previously conditioned Purification Plate. The entire process is done after one additional centrifugation step.
The process is applied in plant breeding work in high throughput. Exemplary data from 96-well extractions with a large breeding company are shown below.
DNA is of consistently high purity and reliably detected in qPCR. There are no reaction drop-outs and there is no more need for a re-work on individual reactions!
SupplierBioEcho Life Sciences GmbH
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