Hyperion Imaging Mass Cytometry System - Witec AG

Comprises Hyperion Tissue Imager, Helios Mass Cytometer, Computer and Software

Description

Visualize a new path forward

Changing the course of how diseases are treated and ultimately cured requires a comprehensive understanding of complex cellular phenotypes and their interrelationships in the spatial context of the tissue microenvironment. To meet this great challenge, we developed the Hyperion™ Imaging System.

The Hyperion™ Imaging System brings proven CyTOF® technology together with imaging capability to empower simultanous interrogation of 4 to 37 protein markers using Imaging Mass Cytometry™ (IMC™). Using this system, you can deeply interrogate tissues and tumors at subcellular resolution while preserving the information in tissue architecture and cellular morphology to uncover new biomarker correlations and cell interactions. With the ability to utilize up to 135 channels to detect additional parameters, the Hyperion Imaging System is ideal to meet your image analysis needs today and well into the future.

 

Comprehensive

Highly multiplexed immunohistochemistry (IHC) using proven CyTOF technology enables simultaneous analysis of 4 to 37 protein markers from a single scan

Contextual

Identify new protein biomarkers and deeply profile tissue microenvironments at subcellular resolution while preserving the information in tissue architecture and cellular morphology

Powerful

Preserve precious samples and reduce variability by eliminating dependency on serial sections

 

 

TECHNOLOGY

Imaging Mass Cytometry (IMC) on the Hyperion Imaging System uniquely combines a precisely directed laser beam focused at 1 μm to collect biological samples stained with metal-tagged Maxpar® antibodies and directs these tags for analysis with proven CyTOF technology. Bringing together the high-multiplex capabilities of mass cytometry with imaging using metal-tagged antibodies allows simultaneous interrogation of 4 to 37 protein markers in tissues and tumors at subcellular resolution while preserving the information in tissue architecture and cell morphology. Now researchers can detect discrete signals from each ionized metal tag based on differences in mass instead of wavelength at 1 Da resolution, and in contrast to fluorescence results, get uniform staining with minimal background.

Supplier

Fluidigm Corporation
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Specifications

Channels

135

Mass range

75–209 amu

Abundance sensitivity

0.3% for 159Tb

Frequency

200 pixels/sec

Detection limit

≥400 copies per μm2

Dynamic range

4 orders of magnitude

Calibration

Automated

Operating system

Windows® 7 Pro 64-bit

Data storage

>6 TB RAID (mirrored)

Switch time (between modes)

12 hr

Cross-cell contamination (redeposition)

≤2% (selected direction)

Crosstalk pixel to pixel

≤15% at 200 pixels/sec

Wet tissue thickness for full ablation

≤7 μm

Addressable sample size on slide

≥15 mm x 45 mm

Optical view of sample

≥ 250 μm x 250 μm

File type

TXT, multipage TIFF, OME-TIFF, MCD

Scan area

≥1 mm2/2 hr (@200 Hz)

Hyperion Tissue Imager Dimensions*

Width 56 cm (22 in)

Height 134 cm (53 in)

Depth 56 cm (22 in)

Weight 159 kg (350 lb)

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