Adherent Cell Electrode, 5 mm gap
Description
Reusable plate electrode designed to directly electroporate adherent cells or electroporate cultured cell monolayers on filters.
The Adherent Cell electrode is a reusable electroporation applicator designed to fit into each single well of a 12-well plate, 6-well plate or an individual 35 mm diameter Petri dish.
This plate electrode is specially designed to electroporate cultured monolayers of cells on laminin-coated filters, or on 12 mm polycarbonate or polyester transwell filter units (0.4 mm pore size). It can directly electroporate adherent cells such as human corneal keratocytes, Caco-2, dental pulp cells, NC65 and BCEC and MDCK cells.
The Adherent Cell Electrode consists of two parallel plate electrodes, 7 x 19 mm with 5 mm gap spacing between the plates. Each plate electrode has a 0.3 mm height insulated foot at both ends of the bottom. When the electrode is placed in a dish, a foot minimizes the damage on cells. The high voltage cables are connected to a BTX electroporator, and the electrode is brought down onto the filter until it makes contact with the buffer, usually at 1- 2 mm above the filter. A combination of HV and LV pulses may be used. Adherent cell electrodes are compatible with Gemini X2, ECM 830, ECM 2001+, and Legacy ECM 2001 Generators.
SPECIFICATIONS
Generator Compatibility | ECM 830, ECM 2001+, Legacy ECM 2001, and Gemini X2 |
Pulse Length Range | 1 µs to 35 ms |
Voltage Range | 0 to 300 V |
Volume Range | 0.5 to 3.0 ml |
Autoclavable | No |
Field Type | Homogeneous |
Gap Size | 5 mm |
REQUIRED CABLES
Required for connection to ECM 830 and Gemini X2 | |
45-0204 | Adapter Banana Plug Cables, Red and Black |
Required for connection to ECM 2001+ | |
45-0204 | Adapter Banana Plug Cables, Red and Black |
45-0088 | Female/Female Adapter Set for Banana Plug Cables |
45-2057 or 45-2058 | ECM 2001+ HV Output Cable, 5 ft or 10 ft length |
Required for connection to Legacy ECM 2001 | |
45-0204 | Adapter Banana Plug Cables, Red and Black |
45-0088 | Female/Female Adapter Set for Banana Plug Cables |
45-0083 | Coaxial to Banana Plug Cables, Red and Black, 10 ft |