ECM 630 96 WELL HT-100 SYSTEM WITH MONITORING – Witec AG

Art No | 450656
request only
request only

Description

INCLUDED:

ECM 630 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660 , ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope, Cables, 25-Well Plates (2 mm gap, 6X), HT-100 Plate Handler and a Plate Adaptor

Electroporation system primarily used for bacteria and yeast transformation. High throughput (25- and 96-well) models available.

The ECM 630 is an exponential decay wave electroporation generator providing a broad range of voltage and time constants for full flexibility in varying transfection applications. The ability to select the resistance and capacitance values, and adjust the range of voltages is the key to achieving the optimal time constants and field strengths needed for efficient transformation of prokaryote and eukaryote transfection. The ECM 630 can perform the widest range of electroporation applications among commercially available electroporation generators. Legacy ECM 630 systems are available while supplies last as we transition to new ECM 630 instruments.

Flexibility is important, so BTX has designed a plug and play system for our ECM 630 system to transition between standard cuvettes and to 25/96-well plates using High Throughput Plate Handlers.

The ECM 630 is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.

NEW ECM 630 FEATURES

  • Large, easy to use color touchscreen interface
  • Preset protocols for transforming the most common bacteria, yeast, and microorganisms
  • High-Throughput Plate Handler setting for automatic processing of up to 96 samples at a time with an HT-200 Plate Handler
  • Create, modify, and store up to 1,000 user defined protocols
  • Enhanced Safety Features: Pre-pulse sample resistance check, arc protection, and over-current pulse abort
  • Data Management: Experimental data log stores parameters of all pulses delivered and every sample resistance for QC and troubleshooting purposes
  • Voltage range of 5 V to 3,000
  • Capacitance range of 25 µF to 3,275 µF in 25 µF steps in LV mode; 10, 25, 35, 50, 60, 75, 85 µF in HV mode
  • Resistance range of 25 ohm to 1,575 ohm in 25 ohm steps.

APPLICATIONS

  • Transformation of bacteria and yeast
  • Transfection of mammalian cells
  • Transfection of plant tissue and plant protoplasts
  • High throughput 25- and 96-well electroporation

HIGH THROUGHPUT (HT) ELECTROPORATION

The High Throughput (HT) 96- and 25-well systems offer multi-well electroporation technology for processing multiple samples in seconds. Using the HT multi-well plates instead of traditional cuvettes, the researcher can transition from a single cuvette to either a 96-well or 25-well electroporation plate by using a plate handler. This increases yields and the number of experiment runs in a single day. Experiments take seconds to run, allowing for quick and efficient optimization of the electrical and biological parameters. Once optimized, samples are rapidly processed increasing yields and saving valuable time and money.

The key to the HT System is the combination of the ECM 630 Generator with the Plate Handler and multi-well plates. The Plate Handler holds the plates and delivers the pulse(s) to the wells using parameters set in the ECM 630 generator. The HT-100 Plate Handler provides manual track switching (45-0412, 45- 0422). New ECM 630 instruments are also compatible with HT-200 plate handlers for automatic processing of up to 96 samples at a time.

MONITORING OPTION

Addition of the ENHANCER 3000 allows the researcher to monitor and track key electrical parameters used in electroporation applications. The electrical pulse data is captured as both a graphic display of the wave form and electrical output values following each experiment.

High Throughput Plate Handler – HT-100 included
Creates a safe and reliable working area for processing electroporation plates

The Electroporation Plate Handler creates a safe and reliable working area for processing electroporation plates. They are ideal for use with the ECM 630, ECM 830 and Gemini X2 systems.

The HT-100 and HT-200 Plate Handlers have pulse switching technology integrated into the package and gold-plated contacts to mate with a 96-well electroporation plate. The plate handler delivers a single pulse to the wells of the plate, column by column, using parameters set in the generator. The HT-100 uses manual track switching, while the HT-200 employs auto-sense track switching. Both plate handlers work with 96-well plates and come with an adapter to accommodate 25-well plates.

WARNING: High Throughput Plate handlers are not for use with ECM 2001+ and ECM 2001 systems.

Features & Benefits

  • Quickly optimize for better gene delivery
  • Process up to 96 wells in seconds
  • Compatible with any electroporation buffer
  • Include connectors for standard pulse generators

Specifications

Display Type

7 inch color touchscreen interface

Power Source Voltage

100 to 240 VAC, 50 to 60 Hz, CAT II Power 350 W (Pulsing), 35 W (Idle)

Fusing

Type 3AG, 5 A, 250 V 1/4 x 1 1/4 in; Type T (time delay)

Operating Temperature

4° C to 40° C

Relative Humidity

20% to 80% RH, non-condensing

Operational Status

Internal self test upon start-up

Programmability

Storage over 1000 Protocols

Input

100 V to 240 VAC Universal

Charge Time

LV < 7 s, HV < 4 s

Arc Control

Yes

Voltage Range

LV Mode 5 – 500 V in 1 V steps; HV Mode 505 – 3000 V in 5 V steps

Capacitance Range

LV Mode 25 µF to 3,275 µF in 25 µF steps; HV Mode 10, 25, 35, 50, 60, 75, 85 µF

Resistance Range

LV Mode 25 ohm – 1,575 ohm in 25 ohm steps; HV Mode 50 ohm – 1,575 ohm in 25 ohm steps

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