ECM 2001+ Electrofusion & Electroporation System – Witec AG

APPLICATIONS

ELECTROFUSION

Fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications are facilitated by the combination of AC and DC wave pulses. Fusion is achieved by the generation of an AC current wave form that generates a benign dielectrophoretic alignment of cells. With a 30 ms switchover time from AC to DC, efficient fusion takes place. After fusion, the AC is reapplied maintaining the cell compression for the rounding off process resulting in a higher number of hybrids.

APPLICATIONS

ELECTROPORATION

Electroporation is a standard method used to transfect mammalian cell lines to express recombinant human proteins which are used for therapeutic purposes. Gene delivery by this method is typically used in transient transfections to study protein expression or to temporarily knockout or “silence” these genes using siRNA. This is used to study gene targeting and function.

Square waveform electroporation is an efficient non-viral method for transfecting genes and other molecules into mammalian cell lines. This technology is commonly used to study gene targeting, function and to understand protein regulation.

Alternatively, adding additional selection steps to isolate stably transfected cells allows for ;integration of a gene into the genome of the cell for long term expression of protein. The use of the ECM 2001+ offers the control needed to adjust electrical settings for optimization of parameters. This system is powerful enough to yield high transfection efficiencies for cell lines and difficult to transfect cell types including stem cells and primary cells. The gentle square wave pulse also allows for high cell viability of these cell types.

PRODUCT CONFIGURATIONS BY APPLICATION

Complete Cell Fusion and Electroporation System

For use in a wide range of cell fusion applications and mammalian cell electroporation, ECM 2001+ Cell Fusion System complete (45-2045) is our most versatile instrument configuration. The ECM 2001+ Cell Fusion system complete comes with a selection of fusion chambers (Microslides, Meander Fusion Chamber, Flat Electrode) as well as safety stand and cuvettes for electroporation.

Hybridoma Production

For use in large volume cell fusion applications such as hybridoma production, the ECM 2001+ generator, 2 ml optimization coaxial chamber, and 9 ml production coaxial chamber are used. This system (98-6169) does not require proprietary fusion medium, but can be used with the Cytofusion Medium C for convenience. Coaxial chambers (47-0030, 47-0020) are also available for purchase separately.

Embryo Manipulation

For use in small volume cell fusion applications such as embryo manipulation, we recommend the ECM 2001+ Embryo Manipulation System (45-2047). In this system the ECM 2001 generator, 0.5 mm gap, 1 mm gap, and 3.2 mm gap microslides, and micrograbber adaper cables are provided.

Mammalian Cell Transfections

Utilizing the ECM 2001+ System Complete, for Cuvettes (45-2046) for standard mammalian cell transfections is simple. The AC feature is turned off and the DC mode is set as a square wave electroporation device. The range of voltages and pulse lengths that can be programmed, coupled with the ability to carry out up to 10 pulses per experiment, make this an extremely versatile system for any lab.

Adherent Cell Transfections

Electroporate adherent cells directly into the dish used for cell growth. The ECM 2001+ coupled with the Petri Pulser electrode or the Petri Dish electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in. The Petri Pulser is ideal for 6-well plates and the Petri Dish Electrode is ideal for 100 mm petri dishes.