Trx-tag Antibody, mAb, Mouse - 40 µg

Art No | A00180-40

Trx-tag Antibody, mAb, Mouse - 40 µg

140.00 CHF

Brochure

Size: 40 µg

Description

This antibody reacts with the Thioredoxin fusion protein from E.coli and human. Not yet tested in other species.

Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA - recommended usage: 0.05-1.0 µg/ml
Western Blot - recommended usage: 1.0 µg/ml
Western Blot - recommended usage: 1:100-1:1,000

Target background

Mouse Anti-Trx-tag Monoclonal Antibody reacts with the 109-aa Trx-tag sequence encoded by the PET-32a expression vectors from Novagen.Mouse Anti-Trx-tag Monoclonal Antibody is suitable for detecting fusion proteins that contain a Trx-tag.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Western blot analysis of Trx-tagged fusion protein using Trx-tag Antibody, mAb, Mouse (GenScript, A00180, 1 µg/ml). The signal was developed with Goat Anti-Mouse IgG (H&L)[HRP] Polyclonal Antibody (GenScript, A00180, 1:50,000) and umiSensor HRP Substrate Kit (GenScript, L00221.

Predicted Size: 18 kD
Observed Size: 18 kD

Western blot analysis of Trx-tagged fusion protein using Trx-tag Antibody, mAb, Mouse (GenScript, A00180, 1 µg/ml). The signal was developed with One-Step WesternTM Basic Kit (mouse) (GenScript, L00205).

Predicted Size: 18 kD
Observed Size: 18 kD

Datasheet

Supplier

Genscript
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Specifications

Host species	Mouse
Immunogen	Purified recombinant Trx-tag fusion protein
Species reactivity	E.coli and human
Conjugate	Unconjugated
Form	Lyophilized
Storage buffer	Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Mouse IgG2a
Clonality	Monoclonal
Clone ID	4C12H10