THE™ His Tag Antibody [Biotin], mAb, Mouse - 100µg

Art No | A00613

THE™ His Tag Antibody [Biotin], mAb, Mouse - 100µg

570.00 CHF

Brochure

Size: 100 µg

Description

GenScript THE™ His Tag Antibody [Biotin], mAb, Mouse specificly recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA - recommended usage: 0.02-0.2 µg/ml
Western Blot - recommended usage: 0.5 -1.0 µg/ml

Target background

THE™ His Tag Antibody [Biotin], mAb, mouse is the biotin conjugated THE™ His Tag Antibody, mAb, mouse (A00186) purified from mice ascites by protein A affinity column.The antibody is suitable for detecting the expression level of His in fusion proteins.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Western blot analysis of His tagged fusion protein (Genscript, M0100) using THE His Tag Antibody [Biotin], mAb, mouse (GenScript, A00613, 1 μg/ml).

The signal was developed with Streptavidin-HRP (GenScript, M00091, 1:10,000) and and LumiSensor HRP Substrate Kit (GenScript, L00221).

Predicted Size: 52 KD
Observed Size: 52 KD

Datasheet

Supplier

Genscript
show all products

Specifications

Host species	Mouse
Immunogen	A synthetic peptide HHHHHH coupled to KLH
Conjugate	Biotin
Form	Lyophilized
Storage buffer	Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Mouse IgG1,κ
Clonality	Monoclonal
Clone ID	6G2A9