cGFP-tag Antibody, mAb, Mouse - 40 µg

Art No | A00185-40

cGFP-tag Antibody, mAb, Mouse - 40 µg

140.00 CHF

Brochure

Size: 40 µg

Description

The antibody reacts with various forms of GFP including eGFP, OFP, and GFPuv.

Applications

The investigator must determine the ideal working concentration for each specific application. GenScript has not yet determined the suitability of this antibody for applications other than those listed below. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and length of incubations.

ELISA - recommended usage: 0.05-1.0 µg/ml
Western Blot - recommended usage: 1.0-2.0 µg/ml
Western Blot - recommended usage: 1:100-1:1,000

Target background

The coral green fluorescent protein (cGFP) from vector pRNATin-H1.2/Neo (GenScript, SD1223) is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. Mouse Anti-cGFP-tag Monoclonal Antibody can be used to detect cGFP fusion protein in Western blot analyses.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Lane 1: OFP transfecting 293 cell lysate
Lane 2: EGFP transfecting 293 cell lysate
Lane 3: 5 ng GFPuv protein
Lane 4: Negative 293 cell lysate

Primary antibody:

A. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (GenScript, A00185)
B. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (X Company)

Secondary antibody:

Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:50,000)

Specificity analysis of THE GFP Antibody, mAb, Mouse (GenScript, A00185) by Western blot using a variety of variants of GFP protein such as eGFP, cGFP and AcGFP1.

The signal was developed with IRDyeTM 800 Conjugated Goat Anti-mouse IgG.

Datasheet

Supplier

Genscript
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Specifications

Host species	Mouse
Immunogen	Purified recombinant cGFP fusion protein
Conjugate	Unconjugated
Form	Lyophilized
Storage buffer	Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 1 mg/ml
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification	Protein G affinity column
Isotype	Mouse IgG2b
Clonality	Monoclonal
Clone ID	5E5G11