Platinum Tweezertrode, 5 mm Diameter (includes 45-0204 cables)
Description
Tweezer-style reusable electrodes for in vivo and in utero applications in animal tissues.
Tweezertrodes electrodes are reusable non-invasive, tweezer-style electrodes for drug or gene delivery in animal tissues.Tweezertrodes may be used for many in vivo applications, including in utero and ex-vivo gene transfection, eletroporation therapy, and transdermal drug delivery.
Tweezertrodes consist of a standard 11.5 cm tweezer that has been modified with stainless steel circular or disk electrodes at the tip. The gap between the electrodes disks may be adjusted from under 1 mm to over 2 cm. Tweezertrodes are available in various sizes and two different metal alloys either platinum or stainless steel. Platinum Tweezertrodes are available in 7 mm, 5 mm, 3 mm and 1 mm diameters and stainless steel are available as either 10 mm or 7 mm diameters.
Triple Tweezertrodes have three electrode contacts with adjustable position and polarities. This type of triple electrode been shown to improve the efficiency of electrical field distribution for in utero electroporation applications.
These electrodes connect to BTX generators using the Tweezertrode Connection cables (45-0204), and are compatible with the ECM 830, ECM 2001+, Gemini X2, and Legacy ECM 2001 generators. The Anode (+, red cable) of the Tweezertrode electrode corresponds to the side of the Tweezertrode with the blue adjusting screw on it.
Tweezertrodes may be cleaned with a mild detergent and sterilized with 70% ethanol or ethylene oxide.
APPLICATIONS & USE
Following localized or systemic injection of the molecule of interest, the Tweezertrode electrode disks are used to grasp the tissue of interest. An electroporation pulse is then applied; initiating pore formation and incorporation of the molecule into the cells of the tissue in direct contact with the electrode disk.
Tweezertrodes can be used to facilitate localized electroporation of various preparations. Application of these electrodes for in utero transfection, transdermal drug delivery and electroporation therapy have been described. Tweezertrodes have proven particular useful for embryonic or even spermatogonial cell electroporation for effective production of transgenic and knockout mice. The design of the Tweezetrodes is also particular suited for zebrafish applications for studies aiming to rapidly
study gene function in whole organism.
REQUIRED CABLES
Required for connection to Gemini X2 and ECM 830 | |
45-0204 | Adapter Banana Plug Cables, Red and Black |
Required for connection to ECM 2001+ | |
45-0204 | Adapter Banana Plug Cables, Red and Black |
45-0088 | Female/Female Adapter Set for Banana Plug Cables |
45-2057 or 45-2058 | ECM 2001+ HV Output Cable, 5 ft or 10 ft length |
Required for connection to Legacy ECM 2001 | |
45-0204 | Adapter Banana Plug Cables, Red and Black |
45-0088 | Female/Female Adapter Set for Banana Plug Cables |
45-0083 | Coaxial to Banana Plug Cables, Red and Black, 10 ft |
Standard Capabilities
Generator Compatibility | ECM 830, ECM 2001, Gemini X2 |
Voltage Range | 0 to 200 VDC (do not use AC current) |
Pulse Length Range | 1 μs to 200 ms |
Pulse Number Range | 1 to 99 (depending on voltage) |
Operating Temperature | 5oC to 40oC |
Intended Use | Indoor use only |
Relative Humidity | 20 to 80% |