Moxi Cyte Viability Reagent (PI) 15 mL

Size: 15 mL

Description

Recommended for use with Moxi V, Moxi Flow and Moxi GO II flow cytometers for rapid, accurate and precise cell viability, cell count, and cell volume measurements.

Propidium Iodide (PI) is a fluorescent dye that impermeable to healthy cells. When cells dye, their membranes become permeable. PI enters the cell, binds to the DNA, and increases fluorescence 50x +. This strong signal for measuring cell viability has made PI the gold-standard approach used in flow cell viability assessment.

Supplier

Orflo Technologies
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Specifications

Applications	Cell Viability
Cassette Types	Type MF-M\|Type MF-S\|Type S+
Compatible Instruments	Moxi V \| Moxi GO II \| Moxi Flow
Formulation	Phosphate-buffered saline, ph 7.4, containing 0.045% sodium azide, surfactant
Number of Tests	~36
Protocol	1a. For starting cell concentration of 0.1-5e6 cells/ml dilute 15 uL of sample with 135 uL of Orflo PI stock solution 1b. For starting cell concentration of 5-10M cells/ml dilute 7.5 uL of sample with 142.5 uL of Orflo PI stock solution 2. Incubate for 5 minutes in the dark at room temperature (25C) 3. Select Viability Assay option on Moxi Flow touch screen 4. Insert cassette, close door and wait for auto-laser alignment to complete 5. Mix final solution by gently inverting tube 5-10x 6. Pipette 75µL of solution into cassette well 7. Close door of Moxi Flow unit 8. Test will run automatically in 8-10 seconds
Sample Type	Mammalian Cells Large Yeast Large Algae Protozoa Primary Mammalian cells\|immortalized adherent cells non-ES cell types
Storage Stability and Handling	Store at 2-8°C