PhaseAll™ RNA Extraction Reagent
Reliable RNA, DNA and protein extraction from a single sample. PhaseAll™ is a powerful phenol/guanidine-based reagent for the simultaneous extraction of RNA, DNA and protein from biological samples.
Designed for robust phase separation and high-yield recovery, PhaseAll™ offers a versatile solution for researchers requiring multiple biomolecules from limited or precious sample material, with this classic tri-extraction formulation.
Can I use PhaseAll™ for miRNA extraction?
Yes. miRNA can be extracted from all solid samples using PhaseAll™ and following the standard RNA isolation protocol described in the product manual. You can modify this protocol for extraction of free miRNAs from blood serum as outlined below:
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Sample Preparation:
Add 50–250 µl of serum to 750 µl PhaseAll™. Gently invert the tube 5–8 times and incubate at room temperature for 15 minutes. -
Phase Separation:
Add 200 µl chloroform, invert for 15 seconds, and incubate again for 5 minutes at room temperature.
Centrifuge at 12,000 × g for 15 minutes at 4 °C. -
RNA Precipitation:
Carefully transfer the upper aqueous phase to a new tube. Add 500 µl isopropanol, incubate for 10 minutes at room temperature, and centrifuge again at 12,000 × g for 10 minutes at 4 °C. -
RNA Washing and Resuspension:
Wash the pellet with 75% ethanol, air-dry at room temperature for 30 minutes, and resuspend in 20 µl RNase-free water. -
RNA Quantification:
Measure RNA concentration and purity using a NanoDrop™ UV-Vis Spectrophotometer at A260/280 nm and A260/230 nm ratios.