FAQs RiboShield™ RNase Inhibitor – Witec AG

How to use RiboShield™ RNase inhibitor?

All PCRBIO products for 1-step and 2-step RT-PCR and RT-qPCR, as well as for cDNA synthesis, already contain the RNase inhibitor, so there is no need to add RiboShield™ RNase inhibitor when using these products.

If used with other kits, we recommend adding 1µL (40U) of RiboShield™ RNase inhibitor in a reaction mix of 20µL. In case the source of RNA is known to be rich in RNases, a titration may be required to verify full protection of the ribonucleic acid.

What can RiboShield™ RNase inhibitor be used for?

RiboShield™ RNase inhibitor is designed for use in RNA-sensitive applications such as RT-qPCR, cDNA synthesis and RNA-seq, where the presence of even small amounts of RNase can be detrimental to RNA quality and experimental outcome. In addition, it can be added to RNA extraction buffers to protect the ribonucleic acid from degradation at the first step of isolation.

What does RiboShield™ RNase inhibitor do?

RiboShield™ RNase inhibitor acts as a shield against the degradation of target RNA which can be caused by ribonuclease contamination of the RNA preparation.

What kind of RNases can be inhibited using RiboShield™ RNase inhibitor?

RiboShield™ RNase inhibitor is a protein-based inhibitor that non-covalently binds and blocks the activity of the most common and troublesome RNases, including eukaryotic RNases of the neutral type (e.g. RNases A, B and C). It does not inhibit RNases T1, T2, U1, U2, CL3, RNase I and H.