detect
physiological parameters of living cells and tissue slices in a label-free and
non-invasive assay. Cells are placed on a sensor chip and supplied with medium
in a perfusion system. The multiparametric sensor chip continuously measures:
·
the
oxygen consumption
·
the
extracellular acidification
Both
parameters determine the acute rates of the cellular energy metabolism thus
providing essential information on the cellular status and the changes thereof
in response to external stimuli
·
the
cell impedance
The
cell impedance detects alterations in the cellular adhesion/confluence and
morphology
The
Bionas®
analyzing system is
available with 1, 2, 6 parallel Biomodules
.
Long-term
studies and regeneration
A
perfusion system allows for parameter recordings over several days and makes
it possible for the first time:
·
to
monitor cellular responses to drug exposure in long-term studies
·
to
estimate recovery afterwards for the same cell population
·
to
collect information on adaptation effects after repeated drug exposure
Bioenergetics
+ cell morphology
The parameter profiles consistently reflect drug induced responses of the acute cellular energy metabolism and corresponding alterations in cell morphology (cell-cell contacts, cell-matrix interactions, ligand binding to receptors).
 |
 |
|
Fig. 1 AAP exposed primary human hepatocytes with remarkable changes in metabolic rates while cell impedance remains unaffected. After removal of AAP cells recovered completely. |
Fig. 2 Diclofenac caused responses in HepG2 cells showing non-linear changes in bioenergetics and cell impedance. These effects are irreversible. |