Introducing the Superior Sample Preparation System for Next-Generation Sequencing

The Access Array™ Integrated Fluidic Circuit (IFC) facilitates parallel amplification of 48 unique samples, in effect preparing 48 sequencing libraries, in just a few hours. Every reaction combines both an amplicon tagging and a barcoding (identification) step that enables all 48 amplicons to be multiplexed at the sequencing step. This powerful chemistry simplifies the up-front preparation and maximizes the utility of today’s next generation sequencers.

The Fluidigm Access Array System can be used with any PCR-based sample preparation method and with the reagents and primers of your choice. The system includes Access Array IFCs—single-use microfluidic chips—two IFC Controller AXs, and the Stand-Alone Thermal Cycler that easily deliver sample throughput and coverage that scale with laboratory needs.

Key Features:

Sample Capture and Target Enrichment
Only sequence areas of interest.

Sample Barcoding for Multiplexed Sequencing
Sequence multiple samples per run.

Sequencing Library Preparation Using Amplicon Tagging
Eliminate traditional library preparation.

Key Benefits:

• High Throughput – Enriched targets from 48 samples per chip.
• Easy-to-Use – Only six manual steps required to produce 2,304 amplicons.
• Time efficient – Prepares 48 libraries in a few hours at minimal cost.

Chips

48.48 Access Array IFC

Instruments

IFC Controller AX

The Fluidigm IFC Controller AX is designed to prime and load 48.48 Access Arrays exclusively. One chip at a time, the AX sets up 2,304 reactions quickly and easily.

Key advantages include:

• Automated setup of 48.48 Access Array IFCs
• Significantly fewer liquid handling steps compared to conventional platforms
• Self-contained and fully integrated design
• Touch screen interface

Fluidigm IFC Cycler

The Fluidigm IFC Cycler is specially designed for thermal cycling of Fluidigm dynamic and digital arrays.

Key advantages include:

• Highly efficient workflow for all applications
• Easy scalability to meet throughput requirements
• Familiar, easy to use interface

Product Literature

• 48.48 Access Array IFC - Sample Preparation for Next Gen Sequencing
  

Sample Capture and Target Enrichment

Sample capture and target enrichment refer to the ability to select a specific region of interest prior to sequencing. For example, if you were interested in examining 20 specific genes from a large cohort of individuals it would be both wasteful and prohibitively expensive to sample the entire genome of each individual. Instead, sample capture and target enrichment technologies allow you to select the specific areas of interest from each individual and thus only sequence that specific area of interest.

Sample Barcoding for Multiplexed Sequencing

One of the largest challenges facing next-generation sequencing operators today is how to utilize the massive amounts of throughput enabled by the new crop of instruments. While all of the systems in use today allow massive amounts of data to be generated on a per sample basis, they lack a simple and reliable method of running multiple samples per run, and thus harvesting the tremendous throughput of these instruments. Barcoding samples during the sample capture process enables the users to combine multiple samples per sequencing run, and then later during the data analysis step, determine which sample the resulting data came from.

Sequencing Library Preparation Using Amplicon Tagging

Library preparation for next-generation sequencing is by far the most time and labor demanding part of the entire next-generation sequencing process. While necessary for whole genome sequencing studies, the process can be almost entirely eliminated for deep resequencing projects through the use of amplicon tagging. By incorporating the adaptor sequences into the primer design the final PCR product is ready to go into emPCR or onto the flowcell since it already contains the necessary capture sequences.